Best Upsets are marked with an asterisks*. Some teams are under-rated due to having limited quality wins.
| – | Team | Rank | VS | Rank | Opp Team | Details |
| 1 | Le Moyne | #1 | 21 – 13 | #10 | Pace | 04/24/2021 |
| 2 | Queens | #3 | 18 – 14 | #19 | Limestone | 04/25/2021 |
| 3 | West Chester | #4 | 19 – 5 | #28 | Bloomsburg | 04/24/2021 |
| 4 | Assumption | #13 | 13 – 10 | #26 | Franklin Pierce | 04/25/2021 |
| 5 | Queens | #3 | 22 – 4 | #41 | UVA Wise | 04/23/2021 |
| 6 | UIndy | #20 | 24 – 5 | #25 | McKendree | 04/24/2021 |
| 7 | Limestone | #19 | 14 – 10 | #27 | Wingate | 04/23/2021 |
| 8 | Seton Hill | #9 | 17 – 10 | #39 | Walsh University | 04/23/2021 |
| 9 | Mount Olive | #21 | 23 – 16 | #33 | Belmont Abbey | 04/25/2021 |
| 10 | Adelphi | #23 | 18 – 6 | #34 | New Haven | 04/24/2021 |
| 11 | Georgian Court | #22 | 18 – 8 | #38 | Post | 04/24/2021 |
| 12 | Davenport | #12 | 21 – 5 | #51 | Northern Mich. | 04/25/2021 |
| 13 | Davenport | #12 | 18 – 4 | #51 | Northern Mich. | 04/23/2021 |
| 14 | Mercy | #16 | 15 – 6 | #53 | Molloy | 04/24/2021 |
| 15 | Saint Anselm | #24 | 17 – 7 | #46 | Stonehill | 04/24/2021 |
| 16 | Lindenwood | #2 | 18 – 4 | #73 | Lewis | 04/24/2021 |
| 17 | Saint Leo | #17 | 20 – 9 | #58 | Palm Beach Atl. | 04/24/2021 |
| 18 | Mercyhurst | #31 | 11 – 7 | #47 | Lock Haven | 04/24/2021 |
| 19 | Roberts Wesleyan | #8 | 23 – 2 | #79 | Bridgeport | 04/24/2021 |
| 20 | Belmont Abbey | #33 | 20 – 3 | #57 | Barton | 04/23/2021 |
| 21 | Mount Olive | #21 | 18 – 8 | #70 | North Greenville | 04/23/2021 |
| 22 | Franklin Pierce | #26 | 22 – 12 | #66 | Southern New Hampshire | 04/23/2021 |
| 23 | Bentley | #11 | 16 – 3 | #82 | Saint Michael’s | 04/24/2021 |
| 24 | Shippensburg | #30 | 12 – 11 | #69 | Kutztown | 04/24/2021 |
| 25 | Seton Hill | #9 | 17 – 7 | #92 | Gannon | 04/25/2021 |
