Best Upsets are marked with an asterisks*. Some teams are under-rated due to having limited quality wins.
| – | Team | Rank | VS | Rank | Opp Team | Details |
| 1 | Salisbury | #1 | 18 – 7 | #18 | Roanoke | 04/24/2021 |
| 2 | Brockport | #6 | 17 – 14 | #16 | William Smith | 04/24/2021 |
| 3* | Roger Williams | #29 | 20 – 7 | #15 | Western New England | 04/24/2021 |
| 4 | Wesleyan (CT) | #4 | 12 – 9 | #50 | Trinity | 04/23/2021 |
| 5 | Denison | #22 | 16 – 5 | #58 | DePauw | 04/24/2021 |
| 6 | St. Mary’s (MD) | #21 | 14 – 6 | #61 | Southern Virginia | 04/24/2021 |
| 7 | FDU-Florham | #7 | 16 – 14 | #76 | Albright | 04/24/2021 |
| 8 | Geneseo | #8 | 20 – 3 | #77 | Oswego | 04/25/2021 |
| 9* | St. John Fisher | #51 | 8 – 7 | #44 | Nazareth | 04/24/2021 |
| 10* | Shenandoah | #62 | 22 – 12 | #35 | Lynchburg | 04/24/2021 |
| 11* | Stevens | #53 | 8 – 7 | #47 | York | 04/23/2021 |
| 12 | Tufts | #2 | 18 – 2 | #100 | Bates | 04/24/2021 |
| 13 | Susquehanna | #52 | 12 – 7 | #68 | Drew | 04/24/2021 |
| 14* | Union | #63 | 19 – 13 | #60 | Clarkson | 04/24/2021 |
| 15 | Clarkson | #60 | 19 – 9 | #63 | Union | 04/23/2021 |
| 16 | Gettysburg | #36 | 20 – 3 | #101 | McDaniel | 04/24/2021 |
| 17 | RIT | #32 | 14 – 13 | #105 | Rochester | 04/24/2021 |
| 18* | Rochester | #105 | 16 – 8 | #32 | RIT | 04/23/2021 |
| 19 | Ithaca | #23 | 19 – 9 | #117 | Vassar | 04/24/2021 |
| 20 | Ithaca | #23 | 21 – 13 | #117 | Vassar | 04/23/2021 |
| 21 | St. Lawrence | #28 | 21 – 16 | #116 | Skidmore | 04/24/2021 |
| 22 | St. Lawrence | #28 | 18 – 11 | #116 | Skidmore | 04/23/2021 |
| 23 | Rhodes | #40 | 22 – 7 | #108 | Centre | 04/25/2021 |
| 24 | Mount Union | #26 | 17 – 14 | #128 | Otterbein | 04/24/2021 |
| 25 | Cortland | #12 | 16 – 9 | #148 | New Paltz | 04/24/2021 |
