Best Upsets are marked with an asterisks*. Some teams are under-rated due to having limited quality wins.
| – | Team | Rank | VS | Rank | Opp Team | Details |
| 1* | Syracuse | #3 | 16 – 7 | #2 | Boston College | 04/24/2021 |
| 2 | North Carolina | #1 | 12 – 11 | #6 | Duke | 04/23/2021 |
| 3 | Notre Dame | #5 | 15 – 11 | #32 | Louisville | 04/24/2021 |
| 4 | Stony Brook | #9 | 17 – 10 | #28 | UAlbany | 04/24/2021 |
| 5 | Drexel | #7 | 17 – 7 | #30 | Towson | 04/23/2021 |
| 6* | Vanderbilt | #24 | 10 – 6 | #16 | Temple | 04/25/2021 |
| 7 | Johns Hopkins | #13 | 20 – 13 | #27 | Michigan | 04/24/2021 |
| 8 | Temple | #16 | 13 – 12 | #24 | Vanderbilt | 04/23/2021 |
| 9 | James Madison | #21 | 15 – 9 | #25 | Elon | 04/25/2021 |
| 10 | Hofstra | #19 | 10 – 9 | #30 | Towson | 04/25/2021 |
| 11* | Virginia Tech | #41 | 20 – 15 | #11 | Virginia | 04/24/2021 |
| 12 | Denver | #14 | 19 – 9 | #44 | Villanova | 04/25/2021 |
| 13 | Denver | #14 | 18 – 10 | #44 | Villanova | 04/23/2021 |
| 14 | Richmond | #29 | 15 – 11 | #31 | Davidson | 04/23/2021 |
| 15 | Jacksonville | #15 | 22 – 11 | #47 | Liberty | 04/25/2021 |
| 16 | UMass | #23 | 18 – 13 | #42 | Saint Joseph’s | 04/25/2021 |
| 17 | Loyola | #12 | 17 – 6 | #53 | Furman | 04/25/2021 |
| 18* | Coastal Carolina | #57 | 13 – 12 | #15 | Jacksonville | 04/23/2021 |
| 19 | UConn | #33 | 16 – 12 | #43 | Georgetown | 04/25/2021 |
| 20* | USC | #39 | 16 – 14 | #37 | Arizona State | 04/23/2021 |
| 21 | UConn | #33 | 15 – 14 | #43 | Georgetown | 04/23/2021 |
| 22 | Stanford | #10 | 9 – 7 | #68 | California | 04/24/2021 |
| 23 | Arizona State | #37 | 20 – 11 | #52 | San Diego State | 04/25/2021 |
| 24 | James Madison | #21 | 21 – 8 | #70 | William and Mary | 04/23/2021 |
| 25 | Robert Morris | #38 | 20 – 10 | #54 | Central Michigan | 04/24/2021 |
