| – | Team | Rank | VS | Rank | Opp Team | Details |
| 1 | Seton Hill | #18 | vs | #16 | Frostburg | 04/10/2021 |
| 2 | Coker | #28 | vs | #12 | Limestone | 04/10/2021 |
| 3 | Caldwell | #34 | vs | #15 | Chestnut Hill | 04/10/2021 |
| 4 | North Greenville | #32 | vs | #19 | Belmont Abbey | 04/10/2021 |
| 5 | Florida Southern | #45 | vs | #8 | Tampa | 04/10/2021 |
| 6 | Tusculum | #31 | vs | #25 | Catawba | 04/11/2021 |
| 7 | Davenport | #52 | vs | #5 | UIndy | 04/10/2021 |
| 8 | Assumption | #57 | vs | #2 | Le Moyne | 04/10/2021 |
| 9 | Emmanuel (Ga.) | #50 | vs | #10 | Mount Olive | 04/10/2021 |
| 10 | CSU Pueblo | #37 | vs | #30 | Colorado Mesa | 04/11/2021 |
| 11 | Queens | #20 | vs | #47 | Anderson (SC) | 04/10/2021 |
| 12 | Lincoln Memorial | #67 | vs | #1 | Lenoir-Rhyne | 04/10/2021 |
| 13 | Mars Hill | #59 | vs | #11 | Newberry | 04/10/2021 |
| 14 | CSU Pueblo | #37 | vs | #33 | Westminster | 04/09/2021 |
| 15 | Adams State | #48 | vs | #30 | Colorado Mesa | 04/09/2021 |
| 16 | Maryville | #14 | vs | #65 | Quincy | 04/10/2021 |
| 17 | Adams State | #48 | vs | #33 | Westminster | 04/11/2021 |
| 18 | Rockhurst | #36 | vs | #46 | Lewis | 04/10/2021 |
| 19 | Felician | #62 | vs | #21 | Georgian Court | 04/10/2021 |
| 20 | Pace | #22 | vs | #63 | Franklin Pierce | 04/10/2021 |
| 21 | AIC | #58 | vs | #39 | Southern New Hampshire | 04/10/2021 |
| 22 | Montevallo | #29 | vs | #69 | Shorter | 04/10/2021 |
| 23 | Wilmington | #49 | vs | #54 | Post | 04/10/2021 |
| 24 | Alabama Huntsville | #66 | vs | #44 | Lander | 04/10/2021 |
| 25 | Molloy | #55 | vs | #61 | St. Thomas Aquinas | 04/10/2021 |
