Best Upsets are marked with an asterisks*. Some teams are under-rated due to having limited quality wins.
| – | Team | Rank | VS | Rank | Opp Team | Details |
| 1 | Stevenson | #3 | 11 – 8 | #11 | York | 04/23/2021 |
| 2 | Christopher Newport | #5 | 20 – 14 | #16 | Southern Virginia | 04/24/2021 |
| 3 | Lynchburg | #4 | 23 – 8 | #19 | Colorado College | 04/25/2021 |
| 4* | St. Lawrence | #13 | 17 – 7 | #12 | Ithaca | 04/24/2021 |
| 5 | Lynchburg | #4 | 19 – 6 | #36 | Randolph-Macon | 04/24/2021 |
| 6 | Salisbury | #2 | 13 – 4 | #48 | Saint Mary’s (Md) | 04/24/2021 |
| 7 | Stevens | #17 | 20 – 14 | #33 | Eastern | 04/23/2021 |
| 8 | Catholic | #25 | 15 – 10 | #37 | Scranton | 04/24/2021 |
| 9 | Union | #22 | 16 – 10 | #54 | Clarkson | 04/25/2021 |
| 10 | Muhlenberg | #26 | 14 – 3 | #70 | Washington | 04/24/2021 |
| 11 | Williams | #14 | 16 – 13 | #82 | Wesleyan | 04/24/2021 |
| 12 | Tufts | #6 | 31 – 7 | #93 | Bates | 04/24/2021 |
| 13 | Western New England | #43 | 20 – 10 | #59 | Roger Williams | 04/24/2021 |
| 14 | Cortland | #23 | 16 – 4 | #80 | Brockport | 04/23/2021 |
| 15 | Arcadia | #41 | 14 – 9 | #68 | Delaware Valley | 04/24/2021 |
| 16 | Hampden-Sydney | #9 | 19 – 10 | #100 | Shenandoah | 04/24/2021 |
| 17 | Centre | #28 | 11 – 4 | #84 | Rhodes | 04/25/2021 |
| 18 | Illinois Wesleyan | #31 | 25 – 16 | #92 | Elmhurst | 04/24/2021 |
| 19 | Colby | #40 | 20 – 17 | #94 | Connecticut College | 04/24/2021 |
| 20 | Saint John Fisher | #8 | 20 – 9 | #127 | Nazareth | 04/24/2021 |
| 21 | York | #11 | 17 – 3 | #130 | Albright | 04/24/2021 |
| 22 | Misericordia | #38 | 22 – 6 | #104 | Wilkes | 04/24/2021 |
| 23 | Centre | #28 | 10 – 7 | #117 | Berry | 04/23/2021 |
| 24 | Gettysburg | #7 | 20 – 2 | #139 | McDaniel | 04/24/2021 |
| 25 | Hood | #65 | 12 – 5 | #81 | Lebanon Valley | 04/24/2021 |
