| – | Team | Rank | VS | Rank | Opp Team | Details |
| 1 | Lynchburg | #4 | vs | #1 | Christopher Newport | 03/26/2021 |
| 2 | Hampden-Sydney | #6 | vs | #8 | Washington and Lee | 03/27/2021 |
| 3 | Cabrini | #14 | vs | #3 | Salisbury | 03/27/2021 |
| 4 | DeSales | #15 | vs | #5 | York | 03/27/2021 |
| 5 | Saint John Fisher | #7 | vs | #16 | Ithaca | 03/27/2021 |
| 6 | Southern Virginia | #9 | vs | #20 | Illinois Wesleyan | 03/27/2021 |
| 7 | RIT | #2 | vs | #28 | Clarkson | 03/27/2021 |
| 8 | Transylvania | #11 | vs | #30 | Elmhurst | 03/27/2021 |
| 9 | North Central (Ill.) | #19 | vs | #46 | Marian | 03/27/2021 |
| 10 | Ohio Northern | #38 | vs | #27 | John Carroll | 03/27/2021 |
| 11 | Colorado College | #10 | vs | #60 | Southwestern | 03/26/2021 |
| 12 | Benedictine | #52 | vs | #25 | Carthage | 03/28/2021 |
| 13 | Baldwin Wallace | #13 | vs | #65 | Otterbein | 03/27/2021 |
| 14 | Arcadia | #67 | vs | #12 | Misericordia | 03/27/2021 |
| 15 | Saint Mary’s (Md) | #48 | vs | #42 | Washington | 03/27/2021 |
| 16 | Immaculata | #76 | vs | #14 | Cabrini | 03/27/2021 |
| 17 | Hood | #47 | vs | #44 | Widener | 03/27/2021 |
| 18 | Mount Union | #72 | vs | #39 | Capital | POSTPONED |
| 19 | Centre | #22 | vs | #90 | Birmingham-Southern | 03/28/2021 |
| 20 | Lebanon Valley | #18 | vs | #94 | Alvernia | 03/27/2021 |
| 21 | Centre | #22 | vs | #90 | Birmingham-Southern | 03/27/2021 |
| 22 | Lynchburg | #4 | vs | #112 | Virginia Wesleyan | 03/27/2021 |
| 23 | Berry | #45 | vs | #78 | Hendrix | 03/28/2021 |
| 24 | Guilford | #99 | vs | #24 | Roanoke | 03/27/2021 |
| 25 | Randolph-Macon | #21 | vs | #104 | Bridgewater | 03/27/2021 |
