Correlation between successful isolation of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in cell culture and cycle threshold (Ct) value of quantitative reverse transcription polymerase chain reaction (RT-PCR) targeting E gene suggests that patients with coronavirus disease 2019 (COVID-19) with Ct above 33 to 34 are not contagious and can be discharged from hospital care or strict confinement, according to a brief report published in the European Journal of Clinical Microbiology & Infectious Diseases
This stuff is certainly interesting. Maybe they (labs and CDC, etc) are using this high Ct as a higher standard, which provides them wiggle room to argue they wanted a much higher safety factor? Unfortunately the article cited, implies differently, which appears to skew the numbers we are seeing in favor of the virus still being highly active..and yet, states are relaxing more and more.
There is certainly a disconnect between what we hear and read, vs. what we are seeing.
Help us out here. if Ct value is inverse to a positive, then why is anything greater than 33-34 being used to indicate a positive/contagious person by the CDC/Labs? Based on this data, researchers deduced that patients with Ct values > 34 do not excrete infectious viral particles and thus may be discharged.
Good question. I don't have a good answer. After talking with someone who does RT-qPCR alot, we both just kind of shrugged our shoulders. Perhaps with such high cycle counts they can be certain a false is a false? Err on the side of false positives rather than false negatives? The guidelines show the tests have Level of detection down to 10 virus particle/microliter titers but that is with cycles in the 34 - 36 range depending on machine and extraction kit used. Perhaps this has something to do with it? Sorry I do not have a better answer.
On another interesting nerd note. Here is a preprint of a paper that looks at Ab binding and how to design antibody "cocktails" to take into account "escape" mutations to occur that will effect the binding of a single antibody. https://www.biorxiv.org/content/10.1101 ... 0.292078v1
Multiple by every college in the country then wait for the miracle.
Ain’t nothin’ dumber than an American.
You're so fond of your country and countryman.
TLD’s post is emblematic of the prevailing ethos of the Democratic Party: an elitist view of others, even though its their party with absolutely zero common sense. I’d add they are entirely unpatriotic but that’s too obvious anymore to warrant discussion.
Correlation between successful isolation of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in cell culture and cycle threshold (Ct) value of quantitative reverse transcription polymerase chain reaction (RT-PCR) targeting E gene suggests that patients with coronavirus disease 2019 (COVID-19) with Ct above 33 to 34 are not contagious and can be discharged from hospital care or strict confinement, according to a brief report published in the European Journal of Clinical Microbiology & Infectious Diseases
This stuff is certainly interesting. Maybe they (labs and CDC, etc) are using this high Ct as a higher standard, which provides them wiggle room to argue they wanted a much higher safety factor? Unfortunately the article cited, implies differently, which appears to skew the numbers we are seeing in favor of the virus still being highly active..and yet, states are relaxing more and more.
There is certainly a disconnect between what we hear and read, vs. what we are seeing.
Help us out here. if Ct value is inverse to a positive, then why is anything greater than 33-34 being used to indicate a positive/contagious person by the CDC/Labs? Based on this data, researchers deduced that patients with Ct values > 34 do not excrete infectious viral particles and thus may be discharged.
Good question. I don't have a good answer. After talking with someone who does RT-qPCR alot, we both just kind of shrugged our shoulders. Perhaps with such high cycle counts they can be certain a false is a false? Err on the side of false positives rather than false negatives? The guidelines show the tests have Level of detection down to 10 virus particle/microliter titers but that is with cycles in the 34 - 36 range depending on machine and extraction kit used. Perhaps this has something to do with it? Sorry I do not have a better answer.
On another interesting nerd note. Here is a preprint of a paper that looks at Ab binding and how to design antibody "cocktails" to take into account "escape" mutations to occur that will effect the binding of a single antibody. https://www.biorxiv.org/content/10.1101 ... 0.292078v1
Seems the Ct is all over the place, which really just throws all the resultant testing data we see into one big bucket of uncertainty.
Uncertainty? CDC guidelines have the LoD at 10/microliter in the machines they tested and that was 34 -36 cycles. That is very low count and a rather high cycle count. It would remain to be seen if that low a viral count would be infectious. One thing that the article mentions is not all machines are the same. I know we can run samples on two different machines and have the Ct of one be different than the other. It really is not as black and white as one would want.
Re: All things tRUMPVirus
Posted: Sun Sep 13, 2020 9:54 am
by Brooklyn
Let's put things into prespective
Americans killed in Bowling Green Massacre: 0
Americans killed in Trump's Caravan Attack: 0
Americans killed By Soup Cans: 0
Americans killed by Ebola: 2
Americans killed in Benghazi: 4
Americans killed in the Spanish American: 2,246
Americans killed in War of 1812: 2,260
Americans killed in 9/11/2001 Terrorist Attacks : 2996
Americans killed in American Revolution: 4,435
Americans killed in the Mexican War: 13,283
Americans killed in Korean War: 33,746
Americans killed in Vietnam War: 58,152
Confederate Soldiers Killed on the battle field during the Civil War: 94,000
Union Soldiers Killed on the battle field during the Civil War: 110,100
Americans killed in Europe WWI: 116,516
Americans killed by Japan WWII: 160,914
Americans killed by Trump in tRUMPvirus pandemic: 198,000+
Americans killed by Axis in Europe WWII: 214,362
Small wonder why anti America right wingers take such great pride in Republican death panels and in tRUMP.
Re: All things tRUMPVirus
Posted: Sun Sep 13, 2020 10:04 am
by Peter Brown
Brooklyn wrote: ↑Sun Sep 13, 2020 9:54 am
Let's put things into prespective
Americans killed in Bowling Green Massacre: 0
Americans killed in Trump's Caravan Attack: 0
Americans killed By Soup Cans: 0
Americans killed by Ebola: 2
Americans killed in Benghazi: 4
Americans killed in the Spanish American: 2,246
Americans killed in War of 1812: 2,260
Americans killed in 9/11/2001 Terrorist Attacks : 2996
Americans killed in American Revolution: 4,435
Americans killed in the Mexican War: 13,283
Americans killed in Korean War: 33,746
Americans killed in Vietnam War: 58,152
Confederate Soldiers Killed on the battle field during the Civil War: 94,000
Union Soldiers Killed on the battle field during the Civil War: 110,100
Americans killed in Europe WWI: 116,516
Americans killed by Japan WWII: 160,914
Americans killed by Trump in tRUMPvirus pandemic: 198,000+
Americans killed by Axis in Europe WWII: 214,362
Small wonder why anti America right wingers take such great pride in Republican death panels and in tRUMP.
BLM shot and nearly killed two LA County Sheriffs last night.
Multiple by every college in the country then wait for the miracle.
Ain’t nothin’ dumber than an American.
You're so fond of your country and countryman.
TLD’s post is emblematic of the prevailing ethos of the Democratic Party: an elitist view of others, even though its their party with absolutely zero common sense. I’d add they are entirely unpatriotic but that’s too obvious anymore to warrant discussion.
Actually, it is this:
Petey's post is emblematic of the prevailing ethos of the Republican Party: an elitist view of others, even though its their party with absolutely zero common sense. I’d add they are entirely unpatriotic but that’s too obvious anymore to warrant discussion.
Let us remind everyone that it is the Republican party that is in power.
That it is the Republican party which diminished Obamacare.
That it is the Republican party which stifled the CDC's ability to quarantine virus carriers.
That it is the Republican party which is responsible for the deaths of 195,000+ Americans.
Numbers of Americans dying from tRUMP virus is now roughly 1,000 per day.
By contrast, Canada recently reported ZERO deaths per day (first time since March).
Biggest difference being that the USA is under tRUMP/Republican rule without health care rule while Canada has universal health care and is run by a liberal government.
Bottom line is that the Republican party ruins, runs the USA with absolutely no sense at all. It is totally unpatriotic, totally anti American, and is not the party of moral principle but the party of death and treason.
Peter Brown wrote: ↑Sun Sep 13, 2020 10:04 am
BLM shot and nearly killed two LA County Sheriffs last night.
I watched the video of the attack. But no one has presented any proof or allegation that it was BLM.
So let's see your proof that this group had anything to do with the attack.
Telling that you can't even decry it.
But let's play along, and avoid the awful nature of two people being shot with zero provocation (like what the other Democratic hero, Michael Reinoehl, did).
What do you say then to the BLM supporters and agitators who tried to storm the hospital?
To the protesters blocking the entrance & exit of the HOSPITAL EMERGENCY ROOM yelling "We hope they die" referring to 2 LA Sheriff's ambushed today in Compton: DO NOT BLOCK EMERGENCY ENTRIES & EXITS TO THE HOSPITAL. People's lives are at stake when ambulances can't get through.
Multiple by every college in the country then wait for the miracle.
Ain’t nothin’ dumber than an American.
You're so fond of your country and countryman.
I am fond of the country......the people not so much. You seem to be fond of what the country was and only some of the countrymen.
Very telling.
I don’t lie or tell falsehoods.... I don’t take kindly to stupid. You find your golf ball?
There are very few Americans when you meet them that you can not find common ground with, even bozo Democrats. The fact that you can't see that is why you are a Democrat.
There is a ton of really interesting stuff on this site, albeit rather uber nerdy.
I'm on it. Thanks..
Re: All things tRUMPVirus
Posted: Sun Sep 13, 2020 10:45 am
by Brooklyn
Peter Brown wrote: ↑Sun Sep 13, 2020 10:26 am
Telling that you can't even decry it.
But let's play along, and avoid the awful nature of two people being shot with zero provocation (like what the other Democratic hero, Michael Reinoehl, did).
What do you say then to the BLM supporters and agitators who tried to storm the hospital?
To the protesters blocking the entrance & exit of the HOSPITAL EMERGENCY ROOM yelling "We hope they die" referring to 2 LA Sheriff's ambushed today in Compton: DO NOT BLOCK EMERGENCY ENTRIES & EXITS TO THE HOSPITAL. People's lives are at stake when ambulances can't get through.
Brooklyn wrote: ↑Sun Sep 13, 2020 9:54 am
Let's put things into prespective
Americans killed in Bowling Green Massacre: 0
Americans killed in Trump's Caravan Attack: 0
Americans killed By Soup Cans: 0
Americans killed by Ebola: 2
Americans killed in Benghazi: 4
Americans killed in the Spanish American: 2,246
Americans killed in War of 1812: 2,260
Americans killed in 9/11/2001 Terrorist Attacks : 2996
Americans killed in American Revolution: 4,435
Americans killed in the Mexican War: 13,283
Americans killed in Korean War: 33,746
Americans killed in Vietnam War: 58,152
Confederate Soldiers Killed on the battle field during the Civil War: 94,000
Union Soldiers Killed on the battle field during the Civil War: 110,100
Americans killed in Europe WWI: 116,516
Americans killed by Japan WWII: 160,914
Americans killed by Trump in tRUMPvirus pandemic: 198,000+
Americans killed by Axis in Europe WWII: 214,362
Small wonder why anti America right wingers take such great pride in Republican death panels and in tRUMP.
BLM shot and nearly killed two LA County Sheriffs last night.
Peter Brown wrote: ↑Sun Sep 13, 2020 10:26 am
Telling that you can't even decry it.
But let's play along, and avoid the awful nature of two people being shot with zero provocation (like what the other Democratic hero, Michael Reinoehl, did).
What do you say then to the BLM supporters and agitators who tried to storm the hospital?
To the protesters blocking the entrance & exit of the HOSPITAL EMERGENCY ROOM yelling "We hope they die" referring to 2 LA Sheriff's ambushed today in Compton: DO NOT BLOCK EMERGENCY ENTRIES & EXITS TO THE HOSPITAL. People's lives are at stake when ambulances can't get through.
Let's have your "proof" that a member of BLM shot those 2 sheriffs as you claimed.
We'll wait, again ...
And the hospital protesters?
Re: All things Chinese CoronaVirus
Posted: Sun Sep 13, 2020 2:52 pm
by Typical Lax Dad
We are likely to hit 200,000 dead Americans from Coronavirus on Wednesday.
“We are going to be down to close to zero”
Re: All things Chinese CoronaVirus
Posted: Sun Sep 13, 2020 3:25 pm
by CU88
DEPLORABLE, politics before health and science
Political appointees at the Department of Health and Human Services have sought to change, delay and prevent the release of reports about the coronavirus by the Centers for Disease Control and Prevention because they were viewed as undermining President Trump’s message that the pandemic is under control.
Michael Caputo, the top HHS spokesman, said in an interview Saturday that he and one of his advisers have been seeking greater scrutiny of the CDC’s weekly scientific dispatches, known as the Morbidity and Mortality Weekly Report, for the past 3½ months. The adviser, Paul Alexander, has sent repeated emails to the CDC seeking changes and demanding that the reports be halted until he could make edits.
The emails, first reported late Friday by Politico, describe the CDC documents, widely known as the MMWR, as being “hit pieces on the administration.” Caputo confirmed the authenticity of the emails.